8,598 research outputs found
Frequency doubling in the cyanobacterial circadian clock
Organisms use circadian clocks to generate 24-h rhythms in gene expression. However, the clock can interact with other pathways to generate shorter period oscillations. It remains unclear how these different frequencies are generated. Here, we examine this problem by studying the coupling of the clock to the alternative sigma factor in the cyanobacterium . Using single-cell microscopy, we find that , a key photosynthesis gene regulated by both and the clock, is activated with two peaks of gene expression every circadian cycle under constant low light. This two-peak oscillation is dependent on , without which rhythms revert to one oscillatory peak per day. We also observe two circadian peaks of elongation rate, which are dependent on , suggesting a role for the frequency doubling in modulating growth. We propose that the two-peak rhythm in expression is generated by an incoherent feedforward loop between the clock, and . Modelling and experiments suggest that this could be a general network motif to allow frequency doubling of outputs.This research was made possible by the award of a European Research Council under the European Unionâs Seventh Framework Programme (FP/2007-2013)/ERC Grant Agreement 338060. The work in the Locke laboratory is further supported by a fellowship from the Gatsby Foundation (GAT3272/GLC) and a Fellowship from the Human Frontier Science Program (CDA00068/2012)
Patterns of Individual Shopping Behavior
Much of economic theory is built on observations of aggregate, rather than
individual, behavior. Here, we present novel findings on human shopping
patterns at the resolution of a single purchase. Our results suggest that much
of our seemingly elective activity is actually driven by simple routines. While
the interleaving of shopping events creates randomness at the small scale, on
the whole consumer behavior is largely predictable. We also examine
income-dependent differences in how people shop, and find that wealthy
individuals are more likely to bundle shopping trips. These results validate
previous work on mobility from cell phone data, while describing the
unpredictability of behavior at higher resolution.Comment: 4 pages, 5 figure
Dark halo baryons not in ancient halo white dwarfs
Having ruled out the possibility that stellar objects are the main
contributor of the dark matter embedding galaxies, microlensing experiments
cannot exclude the hypothesis that a significant fraction of the Milky Way dark
halo might be made of MACHOs with masses in the range 0.5-0.8 \msun. Ancient
white dwarfs are generally considered the most plausible candidates for such
MACHOs. We report the results of a search for such white dwarfs in a proper
motion survey covering a 0.16 sqd field at three epochs at high galactic
latitude, and 0.938 sqd at two epochs at intermediate galactic latitude (VIRMOS
survey), using the CFH telescope. Both surveys are complete to I = 23, with
detection efficiency fading to 0 at I = 24.2. Proper motion data are suitable
to separate unambiguously halo white dwarfs identified by belonging to a non
rotating system. No candidates were found within the colour-magnitude-proper
motion volume where such objects can be safely discriminated from any standard
population as well as from possible artefacts. In the same volume, we estimate
the maximum white dwarf halo fraction compatible with this observation at
different significance levels if the halo is at least 14 gigayears old and
under different ad hoc initial mass functions. Our data alone rules out a halo
fraction greater than 14% at 95% confidence level. Combined with two previous
investigations exploring comparable volumes pushes the limit below 4 % (95%
confidence level) or below 1.3% (64% confidence), this implies that if baryonic
dark matter is present in galaxy halos, it is not, or it is only marginally in
the form of faint hydrogen white dwarfs.Comment: accepted in Astronomy and Astrophysics (19-05-2004
Burgers velocity fields and dynamical transport processes
We explore a connection of the forced Burgers equation with the
Schr\"{o}dinger (diffusive) interpolating dynamics in the presence of
deterministic external forces. This entails an exploration of the consistency
conditions that allow to interpret dispersion of passive contaminants in the
Burgers flow as a Markovian diffusion process. In general, the usage of a
continuity equation , where
stands for the Burgers field and is the
density of transported matter, is at variance with the explicit diffusion
scenario. Under these circumstances, we give a complete characterisation of the
diffusive matter transport that is governed by Burgers velocity fields. The
result extends both to the approximate description of the transport driven by
an incompressible fluid and to motions in an infinitely compressible medium.Comment: Latex fil
Development of an invasively monitored porcine model of acetaminophen-induced acute liver failure
Background: The development of effective therapies for acute liver failure (ALF) is limited by our knowledge of the pathophysiology of this condition, and the lack of suitable large animal models of acetaminophen toxicity. Our aim was to develop a reproducible invasively-monitored porcine model of acetaminophen-induced ALF.
Method: 35kg pigs were maintained under general anaesthesia and invasively monitored. Control pigs received a saline infusion, whereas ALF pigs received acetaminophen intravenously for 12 hours to maintain blood concentrations between 200-300 mg/l. Animals surviving 28 hours were euthanased.
Results: Cytochrome p450 levels in phenobarbital pre-treated animals were significantly higher than non pre-treated animals (300 vs 100 pmol/mg protein). Control pigs (n=4) survived 28-hour anaesthesia without incident. Of nine pigs that received acetaminophen, four survived 20 hours and two survived 28 hours. Injured animals developed hypotension (mean arterial pressure; 40.8+/-5.9 vs 59+/-2.0 mmHg), increased cardiac output (7.26+/-1.86 vs 3.30+/-0.40 l/min) and decreased systemic vascular resistance (8.48+/-2.75 vs 16.2+/-1.76 mPa/s/m3). Dyspnoea developed as liver injury progressed and the increased pulmonary vascular resistance (636+/-95 vs 301+/-26.9 mPa/s/m3) observed may reflect the development of respiratory distress syndrome. Liver damage was confirmed by deterioration in pH (7.23+/-0.05 vs 7.45+/-0.02) and prothrombin time (36+/-2 vs 8.9+/-0.3 seconds) compared with controls. Factor V and VII levels were reduced to 9.3 and 15.5% of starting values in injured animals. A marked increase in serum AST (471.5+/-210 vs 42+/-8.14) coincided with a marked reduction in serum albumin (11.5+/-1.71 vs 25+/-1 g/dL) in injured animals. Animals displayed evidence of renal impairment; mean creatinine levels 280.2+/-36.5 vs 131.6+/-9.33 mumol/l. Liver histology revealed evidence of severe centrilobular necrosis with coagulative necrosis. Marked renal tubular necrosis was also seen. Methaemoglobin levels did not rise >5%. Intracranial hypertension was not seen (ICP monitoring), but there was biochemical evidence of encephalopathy by the reduction of Fischer's ratio from 5.6 +/- 1.1 to 0.45 +/- 0.06.
Conclusion: We have developed a reproducible large animal model of acetaminophen-induced liver failure, which allows in-depth investigation of the pathophysiological basis of this condition. Furthermore, this represents an important large animal model for testing artificial liver support systems
Cross-cultural adaptation and validation of the âspinal cord injury-falls concern scaleâ in the Italian population
Study design: Psychometrics study. Objective: The objective of this study was to develop an Italian version of the Spinal Cord Injury-Falls Concern Scale (SCI-FCS) and examine its reliability and validity. Setting: Multicenter study in spinal units in Northern and Southern Italy. The scale also was administered to non-hospitalized outpatient clinic patients. Methods: The original scale was translated from English to Italian using the âTranslation and Cultural Adaptation of Patient-Reported Outcomes Measuresâ guidelines. The reliability and validity of the culturally adapted scale were assessed following the âConsensus-Based Standards for the Selection of Health Status Measurement Instrumentsâ checklist. The SCI-FCS-I internal consistency, inter-rater, and intra-rater reliability were examined using Cronbachâs alpha coefficient and the intraclass correlation coefficient, respectively. Concurrent validity was evaluated using Pearsonâs correlation coefficient with the Italian version of the short form of the Wheelchair Use Confidence Scale for Manual Wheelchair Users (WheelCon-M-I-short form). Results: The Italian version of the SCI-FCS-I was administered to 124 participants from 1 June to 30 September 2017. The mean ± SD of the SCI-FCS-I score was 16.73 ± 5.88. All SCI-FCS items were either identical or similar in meaning to the original versionâs items. Cronbachâs α was 0.827 (p < 0.01), the inter-rater reliability was 0.972 (p < 0.01), and the intra-rater reliability was 0.973 (p < 0.01). Pearsonâs correlation coefficient of the SCI-FCS-I scores with the WheelCon-M-I-short form was 0.56 (p < 0.01). Conclusions: The SCI-FCS-I was found to be reliable and a valid outcome measure for assessing manual wheelchair concerns about falling in the Italian population
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